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Diagnostics KIT

ELISA -Kit for Detection of Cucumber Mosaic Virus
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) ofCucumber mosaic virus (CMV) were used in ELISA to detect both the subgroup CMV in crude extract. The conserved CP gene of CMV was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect CMV viruses.

Salient Feature

Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of CMV in various crops. The diagnostic kit will help in developing resistant cultivars.

ELISA-Kit for Detection of Papaya ring
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) ofPapaya ring spot virus (PRSV) were used in ELISA to detect potyviruses in crude extract. The conserved CP gene of PRSV was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of poty viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect potyviruses.

Salient Feature

The ELISA kit is sensitive to detect tospovirus infection in crude leaf extract. The sensitivity limit is about 10-25 ng of virus.The kit has been validated by testing a large number of field samples. Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of PRSV and related viral diseases. The diagnostic kit will help in developing resistant cultivars.

ELISA-Kit for Diagnosis of Peanut Mottle Virus
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) of PeMoV were used in ELISA to detect the virus in crude extract. The conserved CP gene of PeMov was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect PeMoV virus

Salient Feature

The ELISA kit is sensitive to detect tospovirus infection in crude leaf extract.The sensitivity limit is about 10-25 ng of virus. The kit has been validated by testing a large number of field samples. Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of PeMoV. The diagnostic kit will help in developing resistant cultivars.

ELISA-Kit for Detection of Tospo Virus
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) ofGroundnut bud necrosis virus (GBNV) were used in ELISA to detect tospoviruses in crude extract. The CP gene of GBNV was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect tospoviruses under serogroup IV.

Salient Feature

The ELISA kit is sensitive to detect tospovirus infection in crude leaf extract. The sensitivity limit is about 25 ng of virus.The kit has been validated by testing over 300 field samples of peanut, tomato, chilli and cucurbits. Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of tospoviral diseases. The diagnostic kit will help in developing resistant cultivars.