Membership Form
Diagnostics KIT

PLANT VIRUS DETECTION KIT
Description

In the present innovation, recombinant fusion proteins were used as antigens to raise polyclonal antibodies for plant viruses. Polyclonal antibody (PAb) generated using the classical approach of antigen preparation, is difficult to be reproduced if the original virus culture is lost. The recombinant coat protein constructs can be stored and maintained for longer periods and can be used for antibody production whenever required.
The expression construct of different viruses (PVX, PVS, PVM, PVY, PVA, BBTV, BSMyV, ToLCNDV, CMV, BBrMV, ORSV, CyMV, GLRaV-3 and GLRaV-4) were developed at the Division of Plant Pathology and were provided to Imgenex, Bhubaneswar to raise the antiserum against the constructs.
Purified recombinant coat proteins of viruses (200 µg) were used to prepare polyclonal antiserum in New Zealand white rabbits and monoclonal antibodies in Swiss albino mice.
Antibodies with good titre against PVX, PVS, PVM, PVY, ToLCNDV, CMV, BBrMV, ORSV, CyMV, GLRaV-3, and GLRaV-4) were used for the preparation of ELISA kits.

Salient Feature

It is an indigenous technology and the market price of the product will be about 50% of the price of the currently available diagnostic reagents and kits, which at present are largely imported.
It is economical as compared to the other available diagnostic kits available in the market. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of virus in various crops and also will help in developing resistant cultivars.

SOIL TEST FERTILIZER RECOMMENDATION METER (STFR)
Description

To increase the cost of production, farm profits and soil health. IARI has developed a digital tool, named Pusa Soil Test and Fertilizer Recommendation (STFR) Meter.
This tool analyzes as many as fourteen soil parameters i.e., soil reaction (pH), lime requirement for acid soil, gypsum requirement for alkali soil, salt content (EC), Organic carbon, available nitrogen, phosphorus and available potassium, available Sulphur, available boron, available zinc, copper, iron and manganese.
It is a programmable colorimeter and analyses soil parameters quantitatively, thus its accuracy is better than other soil test kits which analyze qualitatively based on visual comparison of colours.
Additionally, it gives crop- specific fertilizer recommendation for about 100 crops including field crops, horticultural crops and spices.
It is highly useful for the areas where soil testing facility is not available.
With two days’ training Pusa STFR Meter can be used for soil testing by the farmers themselves. It can be used by village panchayat, Agri –input dealers and several self-help groups working in village for soil testing purpose
It can be used by educated unemployed youths for taking up soil testing as an Agri-enterprise. It is portable and can be operated both by battery and as well as by electricity. It can be connected to PC through computer-interface and results can be communicated to the farmers through instant SMS on a pre-registered cell phone. It can be connected with laptop/computer to generate soil health card.

Salient Feature

It is highly useful particularly for the areas where soil testing facility is not available. The present innovative technology would serve as robust complement to the existing STL network.
It is a low cost, user friendly, digital embedded system and programmable instrument. Pusa STFR Meter consists of a meter, a mini shaker, a reagent-kit (for 50 samples) and other important accessories needed for soil testing.

PCR-BASED DETECTION KIT FOR POMEGRANATE BACTERIAL BLIGHT
Description

The PCR detection kit is specific to Xanthomonas axonopodispv. punicae causing bacterial blight of pomegranate. The kit will amplify a 491 bp region from gyrB gene of the bacterial blight pathogen.
Bacterial blight, caused by Xanthomonas axonopodispv. punicae is a major disease of pomegranate. This detection kit will help to confirm the presence of pathogen in the planting materials.

Salient Feature

The PCR based kit could detect the presence of pathogen in affected pomegranate samples within 3hr, at a detection limit 0.1 ng µl-1 templates DNA with >90% success rate.

ELISA -Kit for Detection of Cucumber Mosaic Virus
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) ofCucumber mosaic virus (CMV) were used in ELISA to detect both the subgroup CMV in crude extract. The conserved CP gene of CMV was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect CMV viruses.

Salient Feature

Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of CMV in various crops. The diagnostic kit will help in developing resistant cultivars.

ELISA-Kit for Detection of Papaya ring
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) ofPapaya ring spot virus (PRSV) were used in ELISA to detect potyviruses in crude extract. The conserved CP gene of PRSV was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of poty viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect potyviruses.

Salient Feature

The ELISA kit is sensitive to detect tospovirus infection in crude leaf extract. The sensitivity limit is about 10-25 ng of virus.The kit has been validated by testing a large number of field samples. Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of PRSV and related viral diseases. The diagnostic kit will help in developing resistant cultivars.

ELISA-Kit for Diagnosis of Peanut Mottle Virus
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) of PeMoV were used in ELISA to detect the virus in crude extract. The conserved CP gene of PeMov was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect PeMoV virus

Salient Feature

The ELISA kit is sensitive to detect tospovirus infection in crude leaf extract.The sensitivity limit is about 10-25 ng of virus. The kit has been validated by testing a large number of field samples. Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of PeMoV. The diagnostic kit will help in developing resistant cultivars.

ELISA-Kit for Detection of Tospo Virus
Description

Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) ofGroundnut bud necrosis virus (GBNV) were used in ELISA to detect tospoviruses in crude extract. The CP gene of GBNV was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect tospoviruses under serogroup IV.

Salient Feature

The ELISA kit is sensitive to detect tospovirus infection in crude leaf extract. The sensitivity limit is about 25 ng of virus.The kit has been validated by testing over 300 field samples of peanut, tomato, chilli and cucurbits. Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of tospoviral diseases. The diagnostic kit will help in developing resistant cultivars.

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