Page 107 - WINGS OF AGRI-INNOVATIONS

66. Insecticidal Formulation Of Novel Strain Of Bacillus Thuringensis Ak 47. 67. A Cross Flow Flexible Membrane Filtration Assembly For Small Processing Volume
Patent Number 340541
Date Of Certificate Issue 06/07/2020 Patent Number 343546
Post Grant Journal Date 10/07/2020 Date Of Certificate Issue 06/08/2020
Publication Number 09/2016 Post Grant Journal Date 14/08/2020
Publication Date 26/02/2016 Publication Number 42/2013
Publication Type INA Publication Date 18/10/2013
Application Number 2361/DEL/2014 Publication Type Ina
Application Filing Date 20/08/2014 Application Number 3771/Del/2012
Field Of Invention BIOTECHNOLOGY Application Filing Date 07/12/2012
Classification (IPC) C12N15/09 Field Of Invention Mechanical engineering
Inventor ANIL KUMAR SAXENA, JASDEEP CHATRATH PADARIA, GOVIND Classification (IPC) B41d
TIKARAMSA GUJAR, AJAR NATH YADAV, SHOWKAT AHMAD Inventor DR. GOPAL P. AGARWAL, MUTHUMAREESWARAN
LONE, MONIKA TRIPATHI, MAHENDRA VIKRAM SINGH M. R, SATYENDRA SINGH
RAJAWAT Abstract:

Abstract: A cross-flow fluid filtration mountable membranes assembly for concentrating and separating the liquid
A formulation of novel strain of Bacillus thuringiensis having insecticidal activity was isolated following stream at low processing volume sample, comprising of flexibility for spiral-wound membranes, Organic
enrichment in sodium acetate buffer amended Luria broth medium from soil of Bhitarkanika mangrove, tubular and Ceramic tubular membrane and 10 means for controlling the pressure, velocity and volume of
Orissa, India. The novel microbe was identified as Bacillus thuringiensis based on 16S rDNA amplification the liquid stream.
and deposited at IMTECH, Chandigarh (accession numbers: MTCC 5846). PCR analysis resulted in successful
amplification of 9 cry and 7 other insecticidal genes. Based on amplification of the conserved domains,
following genes were found to be present in the isolate: cry genes: cryl, 8, crylAb, crylAc, crylC, crylF, crylG,
cry2Aa, cry2Ab, crylAc, other insecticidal, fungicidal and bactericidal genes: vip2A, nheA, nheB, nheC, chit,
chit36 and ZmaR. The strain was found to be effective against lepidopteran pests in bioassay studies. Hence
it is a potential candidate to be employed as a biocontrol agents against insect pests. Moreover, by using
this technology, farmers can save money on chemical pesticides.

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