Page 105 - WINGS OF AGRI-INNOVATIONS

64. Plant Transformation Vector For Suppressing MIPS Gene Expression And Method For Culturing Low
Phytate Soybean
65. Liquid Bioinoculant Of Azotobacter Chroococcum And The Process Thereof
Patent Number 341699
Date Of Certificate Issue 15/07/2020 Patent Number 338098
Post Grant Journal Date 17/07/2020 Date Of Certificate Issue 08/06/2020
Publication Number 06/2017 Post Grant Journal Date 12/06/2020
Publication Date 10/02/2017 Publication Number 48/2012
Publication Type INA Publication Date 30/11/2012
Application Number 2432/DEL/2015 Publication Type INA
Application Filing Date 07/08/2015 Application Number 518/DEL/2011
Field Of Invention BIOTECHNOLOGY Application Filing Date 25/02/2011
Classification (IPC) C12N 15/00 Field Of Invention AGROCHEMICALS

Inventor DR.ARCHANA SACHDEV, MR.AWADHESH KUMAR, MR.ALKESH HADA, Classification (IPC) A01N
MRS.VEDA KRISHNAN, MRS.MONICA JOLLY, MRS. MANSI PUNJABI, Inventor DR. SANGEETA PAUL, DR BISHWAJEET
MRS.NABNEETA BASAK, MS. VANITA PANDEY, MR.ASHISH MARATHE PAUL, MR. MAHESHWAR SINGH RATHI,
DR. BRAHMA KAUSHIK
Abstract:
Plant transformation vector for suppressing MIPS gene expression and method for culturing low phytate Abstract:
soybean provides a plant transformation vector and method for transforming soybean plants. The vector The present invention relates to inexpensive, economical and reliable method of production of liquid
is design to suppress or enhance gene of interest in plants. The plant transformation vector comprises a inoculant with very high population of A. chroococcum of more than 1012 cfu/ml which has a prolonged
plant active promoter, a terminator, a plant selection marker and a bacterial selection marker. The present shelf life of approximately 30 months. This formulation does not need refrigeration for maintaining the
invention also provides a method of transformation for reducing phytate in soybean comprises growing a viability of the bacteria and can be stored at room temperature without affecting the viability of the
soybean plant comprising MlPS”intron hairpin expression cassette operably linked to a promoter {vicilin) bacteria. The formulation can be easily applied on the seed/seedling and does not require preparation of
that drives tissue specific expression in plants. The expression of this novel RNAi hairpin cassette for a sticker solution for its application, it also enhances seed germination, seedling vigor, plant growth and
silencing MIPS gene disclosed here in confers advantageous nutritional properties i.e. reduced phytate yield. The product can be stored in air tight polypropylene bottles, occupies very less space and there is no
content with increased inorganic phosphorous and mineral bioavailability, and leads to valuable and useful production of gas in these bottles. Thus, the product is easy to handle and transport across long distances.
soybean products. The present method is suitable for both small scale and large scale biofertilizer production unit.

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