Page 97 - WINGS OF AGRI-INNOVATIONS

56. Amphiphilic Polymers Based Slow Release Nano Formulations Of β-Carotene And Method Of 57. Fusion Gene Construct For Generation Of Cocktail Antiserum For Detection Of Potyvirus And
Preparation Thereof Cucumovirus
Patent Number 300102 Patent Number 299749
Date Of Certificate Issue 20/08/2018 Date Of Certificate Issue 06/08/2018
Post Grant Journal Date 24/08/2018 Post Grant Journal Date 10/08/2018
Publication Number 33/2014 Publication Number 36/2016
Publication Date 15/08/2014 Publication Date 31/08/2016
Publication Type INA Publication Type INA
Application Number 3744/DEL/2012 Application Number 419/DEL/2012
Application Filing Date 06/12/2012 Application Filing Date 15/02/2012
Field Of Invention POLYMER TECHNOLOGY
Classification (IPC) C08F Field Of Invention BIOTECHNOLOGY
Inventor KUMAR JITENDRA, SHAKIL N.A., SINGH BRAJ BHUSHAN, Classification (IPC) C12N
WALIA SURESH Inventor DR. MANDAL BIKASH, DR. JAIN K. R, MS. KAPOOR REETIKA,
MR. PHANEENDRA C., MS. SWAPNA GEETANJALI
Abstract:
The present technological innovation concentrates on the preparation of Amphiphilic polymers based Abstract:
slow release nano formulations of β-carotene. The formulations are prepared by monolithic dispersion or The present invention relates to the development of dual gene construct for generation of recombinant
encapsulation techniques by utilizing β-carotene, PEG based synthesized amphiphilic polymers and very fusion protein in E. coli and generation of cocktail of polyclonal antibody that will help in simultaneous
little or no solvent. The loading capacity and encapsulation efficiency of developed polymers is reported. detection of Potyviruses (Papaya ringspot virus, PRSV) and Cucumoviruses (Cucumber mosaic virus, CMV).
The formulations are based on both functionalized and non-functionalized amphiphilic polymers imparting The methodology involves cloning of core coat protein genes of PRSV and CMV and preparation of expression
surfactant and carrier properties to the formulation. Because of their universal solubility, formulation construct by placing CMV and PRSV sequence in tandem orientation in pET 28a vector. Cocktail polyclonal
can directly be dissolved in water for application. The developed formulations increase the availability of antibody was generated against the expressed recombinant dual protein of the two viruses. The present
β-carotene for absorption from aqueous solution. The release of β-carotene from these nano formulations invention provides cost and time effective method for simultaneous detection of multiple infection of plant
is also described. The slow release nano formulations of the present invention will help in increasing the viruses as this dual antiserum can be used for simultaneous detection of PRSV and CMV. This strategy would
bioavailability of β-carotene for a longer duration owing to slow release of active molecules. be very useful in detecting viruses in vegetatively propagating crops like Potato or Banana etc.

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