Page 93 - WINGS OF AGRI-INNOVATIONS

52. Development Of Scar Marker For Identification Of Chaetomium Globosum - A Potential Biocontrol 53. Development Of Polymeric Formulations Of Bioactive Molecules And Method Of Preparation Thereof
Agent
Patent Number 294901 Patent Number 295150
Date Of Certificate Issue 26/03/2018 Date Of Certificate Issue 28/03/2018
Post Grant Journal Date 30/03/2018 Post Grant Journal Date 06/04/2018
Publication Number 05/2012 Publication Number 03/2016
Publication Date 03/02/2012 Publication Date 15/01/2016
Publication Type INA Publication Type INA
Application Number 577/DEL/2009 Application Number 3746/DEL/2012
Application Filing Date 25/03/2009 Application Filing Date 06/12/2012
Field Of Invention BIOTECHNOLOGY Field Of Invention POLYMER TECHNOLOGY
Classification (IPC) C12Q1/68 Classification (IPC) C08F
Inventor AGGARWAL RASHMI, GUPTA SANGEETA, RENU
Inventor KUMAR JITENDRA, SHAKIL N.A., ADAK TOTAN,
Abstract: SARKAR DHRUBA JYOTI, ROY CHOWDHURY ARNAB
In this study we report the application of sequence-characterized amplified region (SCAR) marker for the Abstract:
detection of Chaetomium globosum. DNA was extracted from pure cultures of fifteen Chaetomium globosum The present technological innovation concentrates on the preparation of polymers based formulations
isolates (Cg1-Cg15) and one isolates each of C. reflexum (Cr) and C. perlucidum (Cp). 12 URP primers were of agrochemicals for seed quality enhancement and effective pest management by utilizing amphiphilic
used for DNA amplification of all Chaetomium isolates mentioned as above. URP-2R produced a unique polymers and bioactive molecules. The formulations are prepared by monolithic dispersion or encapsulation
DNA band of 1.9 kb in all the isolates of C. globosum but not in C. reflexum and C. perlucidum. This band techniques by utilizing, bioactive molecules, PEG based synthesized amphiphilic polymers and very low
was eluted and ligated into pGEMT vector. Transformed colonies (White) were used for sequencing and solvents or no solvent. The loading capacity and encapsulation efficiency of developed polymers is reported.
four primer pairs were designed. PCR was performed using all the four synthesized Primers. Amplification To ascertain the tenacity properties, the release of bioactive molecules from these formulations in water,
with primer Cg 5P (forward primer) 5”- CAC CAA TCG CAC ACT TTG ACC-3” and Cg2 (2) (reverse primer) is also described. The bioactive molecules, both synthetic and natural, more particularly as imidacloprid,
5”- ACT GAT CGC ACA CTC CAC CTCT -3” produced a unique DNA band of 1.9 kb in all the isolates of thiamethoxam, carbofuran, pendimethalin, sulfosulfuron, and azadirachtin and the likes are used. These
Chaetomium globosum but it was absent in C.perlucidum, C.reflexum, C.cupreum, C. cochlioides as well as formulations of different bioactive materials will release them in controlled manner thereby improving
in other fungal genera Aspergillus flavus, Bipolurissorokiniana and Fusarium moniliformue. Diagnostic PCR their bioefficacy upon the existing formulations. These formulations which provide improved tenacity
was performed using the primer pair IV. The results showed that this SCAR marker can clearly distinguish and uniform coating of a.i therefore minimize the losses during processing / handling. The developed
Chaetomium globosum at inter specific level as well as inter generic level. Our data provided the foundation formulations increase the availability of bioactive materials for protection against various pests in diverse
for a precise and rapid PCR-based diagnostic system for Chaetomium globosum a biocontrol agent at its site use situations. The bioactive molecule which has less water solubility, their solubility in water is improved
of application. by encapsulating them in amphiphilic polymers which facilitate their systemic action. The polymeric
formulations of the present invention will help in protecting an agronomic plant for a longer duration owing
to slow release of active molecules. The developed products may be used as spray and seed treatments for
efficient management of the pests. Additionally, such products are safe to handle and cost effective.

84 85