Page 89 - WINGS OF AGRI-INNOVATIONS

48. Pusa 5Sd-A Bio-Formulation Of Trichoderma Harzianum (IARI P-4) For Seed Treatment 49. A Novel Formulation Of The Plant Growth Promoting Rhizobacteria With Enhanced Shelf-Life And
The Method Of Its Preparation
Patent Number 292555
Date Of Certificate Issue 05/02/2018 Patent Number 292524

Post Grant Journal Date 09/02/2018 Date Of Certificate Issue 02/02/2018
Publication Number 14/2010 Post Grant Journal Date 09/02/2018
Publication Date 02/04/2010 Publication Number 08/2011
Publication Type INA Publication Date 25/02/2011
Application Number 2032/DEL/2008 Publication Type INA
Application Filing Date 28/08/2008 Application Number 1710/DEL/2009
Field Of Invention AGRICULTURE ENGINEERING Application Filing Date 18/08/2009
Classification (IPC) C12N 15/00 Field Of Invention BIOTECHNOLOGY
Inventor DUBEY SUNIL CHANDRA Classification (IPC) C12N 1/20
Inventor LATA, ANUPAMA, PARMAR BALRAJ SINGH
Abstract:
It is an object of this to develop bio-formulation of the potential isolate of Trichoderma harzianium (IARI Abstract:
P-4; MTCC No. 5371) suitable for seed treatment with longer shelf (viability) life. Another objective of this A novel, ecofriendly indigenous, easy to use bacterial inoculant formulation with enhanced shelf-life at
invention is to develop a bio-formulation from the above mentioned fungal antagonist suitable for the otherwise unfavourable high temperatures characteristic of tropics, characterized by one or more strains,
management of diseases of crop plants. In accordance with the present invention, a novel seed dressing species or genera of plant growth promoting microorganisms immobilized and entrapped inside the matrix
bio-formulation “Pusa 5SD” has been developed from the potential isolate of Trichoderma harzianium for of a water insoluble superabsorbent hydrogel of semi-synthetic origin maintained at moisture content
management of soil and seed borne diseases of crop plants. The formulation yielded 1.8 x 106 to 2.1 x 10 equivalent of an appropriate per cent of the water absorption capacity of the superabsorber, capable to
viable cfu/g after 25 months of storage at room temperature and found effective against wilt of chickpea and withstand temperature in the range 0-50°C for an effective time period of 36 to 12 months respectively
dry root rot of chickpea and mungbean. The formulation is also suitable for other species of Trichoderma. with 60-100% survival of the entrapped microbes even in the absence of any extraneous source of
nutrients, providing favourable environment for the conversion of active cells of some stains to transform
into cysts and for others to retain their viability as such, free from microbial attack induced degradation
has been developed by a simple method of introduction of microbes into the carrier matrix under ambient
conditions, with additional advantages of transparent medium for direct examination of microbial cells
under microscope and retaining water in the medium to which it is applied. The formulation can be easily
applied in the soil, soilless media, seed and the like without requiring any chemical reagent.

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