Page 62 - WINGS OF AGRI-INNOVATIONS

22. A Process For The Production Of Blue Green Algal Biofertilizer 23. Efficient Process For The Preparation Of Neem Based Reduced Azadirachtin(S) Pesticides

Patent Number 220746 Patent Number 226204
Date Of Certificate Issue 11/07/2008 Date Of Certificate Issue 15/12/2008

Post Grant Journal Date 25/07/2008 Post Grant Journal Date 02/01/2009
Publication Number 48/2007 Publication Number 41/2007
Publication Date 30/11/2007 Publication Date 12/10/2007
Publication Type INA

Publication Type INA Application Number 1126/DEL/2003
Application Number 444/DEL/2002 Application Filing Date 09/09/2003
Application Filing Date 10/04/2002 Field Of Invention CHEMICAL
Field Of Invention MICRO BIOLOGY Classification (IPC) A01N 43/90
Classification (IPC) A01G 033/00 Inventor SURESH WALIA, VANDANA SHARMA, JITENDRA
KUMAR, BALRAJ SINGH PARMAR
Inventor DR. BRAMHA DUTTA KAUSHIK Abstract:
Abstract: A process for the preparation of tetrahydroazadirachtin, and/or dihydroazadirachtin concentrates from
A process for the production of blue green algal biofertilizer” involves the production of BGA biofertilizer azadirachtin concentrates is described. It is characterized by catalytic reduction of azadirachtin under either
strains individually in the formulated medium by inoculating about 3-10g wet biomass per multiplication of ambient or near ambient conditions of temperature and pressure, under continuous stirring, to yield the
unit with a production of 3-10 days production cycle. The medium formulation is achieved by mixing single claimed product(s) in 80 to 100% yields.
super phosphate, mureate of potash and magnesium sulphate at 0.02-0.09 g/L water, once BGA cultures
grown to a desired biomass say 15-18 g/unit is mixed with wheat/rice straw as carrier at a final population
density of 106 -109 cells per gram carrier containing Aulosira, Tolypothrix, Anabaena and Nostoc strains.
The multiplication units are constructed in a single/double tier inside the glass or polyethylene house.
The multiplication units are made of cement and mortar. In a two-tier system the lower tier is illuminated
by artificial light. The temperature of these units is maintained between 25-45°C during the entire year
by providing heat convertors in winters and exhaust fans with suitable cloth filters in summer. The dried
inoculum containing all the four strains mixed in 1:1:2:2 or 1:1:1:1 (w/w) proportion and packed in suitable
size packets. The shelf life of this inoculum (biofertilizer) is two years if stored in dry and well-ventilated
area. The straw based biofertilizer to be applied by broadcasting immediately after transplantation of paddy
seedlings in a puddled field. Normally 400-g straw-based BGA biofertilizer is enough for 1 acre.

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